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Exploring high‐content screening as a functional genomics tool in biomedicine
Published on 2019-06-28105 Views
High-content phenotypic screening, encompassing both drug and genomic screenings, constitute an invaluable research and discovery tool, which has been extensively explored by the industry and academia
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Presentation
Exploring high content screening as a functional genomics tool in biomedicine00:00
Drug discovery and development01:04
Functional genomics - 101:47
Genome Sequencing - 101:59
The Human Genome at 18 | Feb.2001 Feb.201902:06
The Human Genome in print02:16
The Human Genome in numbers02:21
Genome Sequencing - 202:46
Functional genomics - 202:56
Expression analysis vs functional screening 03:12
Expression analysis vs. functional phenotypic screening04:49
Phenotypic Screening in Functional Genomics - 106:11
Phenotypic Screening in Functional Genomics - 207:39
RNA interference09:05
High Content Screening: getting the whole picture10:58
Automated image analysis - 111:31
Automated image analysis - 212:17
Automated image analysis - 312:20
Automated image analysis - 412:23
Automated image analysis - 512:23
Functional Genomics and RNA based Therapeutics laboratory13:51
Miniaturization : costs and benefits15:00
Video15:32
Functional genomics screening as a tool for the development of novel therapeutics16:03
Functional screening : examples of applications17:31
High Content Screening – How high is high?18:13
Homogeneous assays vs. Microscopy based readouts18:44
High content screening… really?19:27
Image-based cell profiling21:09
High-throughput multiplexed protein readouts25:20
Cell Painting25:23
Identification of host cell factors required for AAV transduction27:19
Adeno-Associated Virus (AAV) vectors27:33
Genome wide siRNA screening for AAV transduction - 128:18
Genome wide siRNA screening for AAV transduction - 228:38
Secondary screening Refinement for AAV specific targets29:55
Genome wide siRNA screening for AAV transduction - 331:27
Secondary screening ɣH2AX foci31:37
DNA Damage Response Top 10 gene targets32:10
Effect of siRNAs on AAV transduction in vivo (liver) Mano32:18
Identification of microRNAs controlling infection by bacterial pathogens34:33
Shigella flexneri and Salmonella Typhimurium35:00
MicroRNAs36:01
Host miRNAs are major regulators of Salmonella and Shigella infection36:29
High-Content Screening: analysis of bacterial infection37:16
Salmonella and Shigella infection are regulated by a different set of miRNAs - 137:28
miRNAs interfere with different stages of Salmonella and Shigella infection38:12
Salmonella and Shigella infection are regulated by a different set of miRNAs - 238:36
Salmonella and Shigella infection are regulated by a different set of miRNAs - 339:08
miR 29b 2 5p increases both early and late steps of Shigella infection - 139:21
miR 29b 2 5p increases both early and late steps of Shigella replication39:44
miR 29b 2 5p increases both early and late steps of Shigella infection - 240:26
miR 29b 2 5p increases filopodia formation and Shigella capture by targeting UNC5C41:17
Suggested reading: Shedding light on microRNA function via microscopy-based screening - 142:03
Suggested reading: Shedding light on microRNA function via microscopy-based screening - 242:32
Suggested reading: Shedding light on microRNA function via microscopy-based screening - 342:43
Suggested reading: Shedding light on microRNA function via microscopy-based screening - 443:05
CRISPR/Cas technology: Precise genome editing and beyond43:47
The CRISPR/ Cas System - 144:00
The CRISPR/ Cas System - 244:27
Nuclease-induced genome editing44:54
CRISPR/Cas system: beyond cutting45:26
CRISPR/Cas system for gene regulation45:51
CRISPR/Cas for disease modelling and large-scale screening47:24
Screening approaches48:14
Thank you48:46