Life beyond the pixels: machine learning and image analysis methods for HCS

author: Peter Horvath, Hungarian Academy of Sciences
published: June 28, 2019,   recorded: May 2019,   views: 78


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In this talk I will give an overview of the computational steps in the analysis of a single cell-based high-content screen. First, I will present a novel microscopic image correction method designed to eliminate vignetting and uneven background effects which, left uncorrected, corrupt intensity-based measurements. I will discuss the Advanced Cell Classifier (ACC) (, a software tool capable of identifying cellular phenotypes based on features extracted from the image. It provides an interface for a user to efficiently train machine learning methods to predict various phenotypes. We developed the Suggest a Learner (SALT) toolbox, which selects the optimal machine learning algorithm and parameters for a particular classification problem. For cases where discrete cell-based decisions are not suitable, we propose a method to use multi-parametric regression to analyze continuous biological phenomena. Finally, to improve the learning speed and accuracy, we recently developed an active learning scheme which automatically selects the most informative cell samples.

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Reviews and comments:

Comment1 Nika, May 17, 2021 at 6:59 p.m.:

Artificial intelligence is now helping us take a global step forward. If you used to have to spend hours in Photoshop to improve the clarity of the image or even remove the background, now you can just go to this site and do it in a couple of clicks

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