Advanced STED microscopy of the membrane organisation in activating T-cells
published: July 9, 2018, recorded: May 2018, views: 477
Report a problem or upload filesIf you have found a problem with this lecture or would like to send us extra material, articles, exercises, etc., please use our ticket system to describe your request and upload the data.
Enter your e-mail into the 'Cc' field, and we will keep you updated with your request's status.
Nanoscale organization of the membranes of living cells plays crucial roles in numerous vital processes, including during the activation of T-cells and their formation of the immunological synapse. However, the detailed nature and function of reorganization of lipids during this key initiating event of immune response remain largely unclear. To gain further insight into the mechanisms, we employed two techniques that probe complementary properties of the membranes at the molecular level: STED-FCS to reveal detailed picture of the diffusion of the lipids, and spectral imaging with environment-sensitive membrane probes, i.e. microspectroscopy, to map differences in local molecular order within the lipid bilayer. We correlated both properties with the locations of the key membrane proteins responsible for triggering the activation of T-cells, such as T-cell receptor. In search for the most sensitive description of the membrane order, we systematically compared the established methods of analysis and representation: generalized polarization, lineshape fitting, and spectral phasors.
Link this pageWould you like to put a link to this lecture on your homepage?
Go ahead! Copy the HTML snippet !