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The appeal of conventional electron microscopy for studying biological systems
Published on 2024-02-1810 Views
UNG
Presentation
The appeal of conventional electron microscopy for studying biological systems00:00
Central Microscopy,Christian-Albrecht University of Kiel - 103:05
Central Microscopy,Christian-Albrecht University of Kiel - 203:34
Unique information provided by Electron Microscopy - 103:57
Unique information provided by Electron Microscopy - 204:19
Unique information provided by Electron Microscopy - 304:31
Increased resolving power of electrons04:37
Beginning of Electron Microscopy - 105:16
Beginning of Electron Microscopy - 205:36
Electron microscopy laid foundations of modern cell biology - 105:45
Electron microscopy laid foundations of modern cell biology - 206:16
Textbook schemes of a cell are based on transmission electron micrographs06:55
Electron Microscopy - possibilities - 107:33
Electron Microscopy - possibilities - 207:45
Light and electron microscopy - 109:08
Light and electron microscopy - 209:40
Beginning of Electron Microscopy10:21
Two types of electron microscopes - 110:43
Two types of electron microscopes - 210:56
Sample interacts with beam electrons11:21
Contrast in Transmission EM - 112:39
Contrast in Transmission EM - 213:14
“Anatomy” of electron microscopes13:47
Negative staining for Transmission EM14:14
Negative staining 15:24
Good preservation15:35
Morphological diversity of phages - 116:15
Morphological diversity of phages - 216:33
Morphological diversity of phages - 316:42
Visualization of plasmid - born archaeal virus MetSV17:02
Life cycle of Bdellovibrio bacterium - 117:42
Life cycle of Bdellovibrio bacterium - 218:16
Life cycle of Bdellovibrio bacterium - 318:38
Sample preparation for electron microscopy18:55
Sample preparation for TEM: resin embedding20:09
Ultra - thin sectioning - 120:42
Ultra - thin sectioning - 221:49
TEM grids,covered with a 50 - nm thin plastic support film22:21
Sample preparation for SEM22:52
Two types of electron microscopy - 123:13
On thin 2D sections, only a small fraction of sample volume is imaged23:41
Two types of electron microscopy - 224:22
Effect of Benzothiazinone and Ethambutol antibiotics - 124:59
Effect of Benzothiazinone and Ethambutol antibiotics - 225:36
Characterization of Bacillus subtilis biofilm26:52
The plastid-nucleus localized DNA-binding protein27:45
Complex samples require combination of imaging at different scales - 129:19
Complex samples require combination of imaging at different scales - 230:54
On-section immunolabelling32:20
Fine Structure - 132:50
Fine Structure - 232:59
Fine Structure - 333:06
Charles Darwin - 133:18
Charles Darwin - 233:28
Charles Darwin - 333:31
Charles Darwin - 433:33
Whole - mount immunofluorescence staining33:48
On - section labeling34:32
Visualization of affinity ligands35:45
Whole - mount fluorescence versus on - section gold labeling36:19
Whole - mount fluorescence versus on - section immunolabeling36:58
The larger the colloidal gold, the weaker the labeling - 137:14
The larger the colloidal gold, the weaker the labeling - 237:32
Labelling density depends on the gold size37:40
Thin sections are suitable for immunofluorescence labelling - 138:05
Enteropathogenic E. coli - 139:42
Enteropathogenic E. coli - 240:14
Cryosectioning for Tokuyasu, thawed cryo-sections40:29
Labelling on Tokuyasu, thawed cryo-sections - 141:15
Labelling on Tokuyasu, thawed cryo-sections - 241:56
Double labeling42:19
Electron detector42:54
Liver tissue: bile canaliculi architecture - 143:11
Liver tissue: bile canaliculi architecture - 244:15
Liver tissue: bulkheads stabilize the tubular shape of bile canaliculi44:51
Mapping fusion and sorting machinery - 146:02
Mapping fusion and sorting machinery - 246:40
Mapping fusion and sorting machinery - 346:52
Confocal microscopy47:01
Single Molecule Localization Microscopy - 147:16
Single Molecule Localization Microscopy - 247:42
Tomography - segmentation of the endosome - 147:53
Tomography - segmentation of the endosome - 248:12
super CLEM workflow - 148:15
super CLEM workflow - 248:19
super CLEM workflow - 348:21
super CLEM workflow - 448:25
super CLEM workflow - 548:28
3 - color superCLEM - 148:30
3 - color superCLEM - 248:38
3 - color superCLEM - 348:40
3 - color superCLEM - 448:41
Cryo EM48:52
Image elements49:27
Cryo EM: Subtomogram averaging increases structural resolution49:44
Cryo electron Tomography - 150:08
Cryo electron Tomography - 250:27
Cryo electron Tomography - 350:53
Cryo electron Tomography - 450:59
Conventional electron microscopy51:35
Untitled52:18