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The tricks neurons use to express their genes: jumping genes, zero-length exons and RNA loops
Published on Sep 07, 20151404 Views
As soon as DNA is transcribed into RNA, various RNA-binding proteins (RBPs) interact with the RNA, and thereby regulate the expression of proteins. This is particularly important in highly polarised c
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Chapter list
The tricks neurons use to express their genes00:00
How are RNAs important for gene expression in neurons?02:27
Measure interactions05:37
Zero-length exons08:04
Splicing makes an mRNA - 108:49
Splicing makes an mRNA - 209:05
Recursive splicing makes same mRNA - 109:11
Recursive splicing makes same mRNA - 209:26
Recursive splicing makes same mRNA - 309:34
Recursive splicing makes same mRNA - 409:37
Genes expressed in the brain are extremely long09:55
Recursive splice sites are present within some of the longest genes10:50
Definition of the cryptic exon is required for recursive splicing12:06
Recursive sites are followed by cryptic poison exons14:37
Recursive site prevents inclusion of the cryptic exon15:38
Suggested model for the mechanism of recursive splicing in vertebrates - 116:48
Suggested model for the mechanism of recursive splicing in vertebrates - 216:58
Suggested model for the mechanism of recursive splicing in vertebrates - 317:03
Suggested model for the mechanism of recursive splicing in vertebrates - 417:07
Suggested model for the mechanism of recursive splicing in vertebrates - 517:12
The cryptic exon is included if preceded by another cryptic exon17:22
Recursive splicing detects upstream cryptic exons - 119:07
Recursive splicing detects upstream cryptic exons - 219:17
Recursive splicing detects upstream cryptic exons - 319:26
Recursive splicing detects upstream cryptic exons - 419:46
Cryptic exon detects other cryptic exons due to recursive splicing20:03
Gene21:12
Jumping genes21:43
Formation of exons from antisense Alu elements22:35
hnRNP C represses splicing of Alu exons24:47
Alu exons can cause human diseases26:21
hnRNP C controls the emergence of Alu-derived cryptic exons27:37
hnRNP C represses damaging cryptic Alu exons29:00
Het29:20
RNA loops30:36
hiCLIP30:40
mRNAs have a structure to form mRNPs31:27
Example 1: RNA structures can act as thermometers33:29
RNA duplexes in 3’ UTR lead to formation of long RNA loops34:22
Long-range RNA duplexes are not computationally predicted35:26
STAU1 regulates cytoplasmic splicing of the XBP1 mRNA36:30
Summary38:21
RBPs regulate the time and space of neuronal gene expression38:56
Thanks to - 140:10
Thanks to - 241:45