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New generation transgenic techniques in rabbits
Published on Sep 04, 20141458 Views
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Chapter list
New generation transgenic techniques in rabbits00:00
Why to use rabbits as model animals00:07
Transgenic rabbit as a bioreactor02:55
First generation transgenic method was microinjection in rabbits04:16
Problems and solutions04:55
Transposon mediated transgenesis05:51
Transzposons are effective weapons in different fields of biotechnology06:24
How it works06:42
The Sleeping Beauty system07:27
Elements of SB-100 System08:37
Microinjection09:30
Results10:39
Determination of integration sites, copy number11:52
Expression12:44
Summary13:15
How to use our GFP rabbit14:08
Designer nuclease technology14:47
ZFN in rabbits- IGM targeted15:13
Our experiences with Zinc finger nuclease in rabbits15:58
Why we have mosaic founders?17:41
TALEN experiments in rabbits18:33
CRISP-R was extremly efficient in rabbits19:37
Targeted insertion is possible but not reported in rabbits20:17
Nuclease technology is the ultimate technique to produce targeted gene modifications20:38
Thank you for attention22:00