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Severity classification of procedures used in experiments on live animals in cancer research
Published on 2014-09-041660 Views
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Severity classification of procedures used in experiments on live animals in cancer research00:00
The choice of experimental models01:02
Definition of "animal model"01:57
Extrapolation from animals to humans02:49
The selection of animal model04:10
Work in cancer research involves05:24
Tumor models in radiobiology06:51
Tumor models and laboratory animals in chemotherapy07:16
Chain of research in translational studies08:15
Models used in preclinical oncology08:40
Laboratory mice09:17
Mice used in cancer research09:23
Inbred immuno competent strains of mice09:37
Nude mice10:41
SCID mice11:49
Transgenic mice12:54
Knockout mice14:04
Mouse tumor models14:36
Transplantable tumor models15:16
Human tumour xenografts - 116:37
Human tumour xenografts - 217:57
Tumor model selection and use - 118:09
Tumor model selection and use - 220:01
Assesment of severity: for each procedure and for the whole project21:01
Severity of procedures; classification of severity22:36
Severity categories23:25
Examples - mild severity procedures24:09
Methods: tumor implantation 25:00
Subcutaneous tumor measurements25:27
Molecular imaging - main fields of usage - implementation of 3Rs‘25:58
Small animal optical bioluminescence imaging - 126:41
Small animal optical bioluminescence imaging - 226:59
Small animal ultrasound - US27:10
Smal animal computed tomography - CT27:19
Small animal magnetic resonance imaging - MRI27:27
Small animal positron emission tomography -PET27:30
Small animal single photon emission computed tomography - SPECT27:35
A combination or accumulation of the following examples may result in a "mild" classification28:08
Examples - moderate severity procedure28:25
Small animal intravital microscopy (IVM)28:50
Intravital microscopy29:03
Examples - moderate severity procedures29:24
Irradiation - moderate procedure29:47
Examples - severe severity procedures30:02
Examples - severe severity classification30:35
Real example - experiment to assess the effectiveness of new compound on tumour growth31:02
Procedures involved in experiment31:57
Real example - experiment to assess the effectiveness of new compound on tumour growth - 132:22
Real example - experiment to assess the effectiveness of new compound on tumour growth - 233:31
Real example - experiment to assess the effectiveness of new compound on tumour growth - 334:09
Real example - experiment to assess the effectiveness of new compound on tumour growth - 435:05
Real example - experiment to assess the effectiveness of new compound on tumour growth - 536:00
Assesment of severity - 136:59
Clinical observation - score tabele - 137:34
Clinical observation - score tabele - 237:59
Score Sheet38:30
Clinical observation - assesment of actual severity39:00
Assesment of severity - 239:46
References and further reading39:58
Thank you for your attention!40:24