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Molecular insights into the mRNA poly(A) tail machinery
Published on Mar 21, 202330 Views
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Molecular insights into the mRNA poly(A) tail machinery00:00
My lab at LMB - 100:06
My lab at LMB - 200:46
My lab at LMB - 300:54
My lab at LMB - 401:02
My lab at LMB - 501:17
My lab at LMB - 602:53
Identification of enzymatic modules in CPF - 104:21
Identification of enzymatic modules in CPF - 204:34
Identification of enzymatic modules in CPF - 304:51
Identification of enzymatic modules in CPF - 405:11
The polymerase module - 107:07
The polymerase module - 208:08
Cryo-EM of the polymerase module08:53
The polymerase module - 109:14
The polymerase module - 209:50
The polymerase module - 310:30
Interaction of Yth1 with the polymerase module11:35
Interaction of Pfs2 with polymerase module12:21
Similarity to other nucleic acid binding complexes - 112:48
Similarity to other nucleic acid binding complexes - 213:57
Pap1 is flexibly tethered - 115:13
Pap1 is flexibly tethered - 215:18
Fip1 is largely disordered17:03
Mapping binding sites on Fip1 - 117:54
Mapping binding sites on Fip1 - 218:24
Mapping binding sites on Fip1 - 318:31
Mapping binding sites on Fip1 - 418:50
Understanding the dynamics of CPF - 119:36
Understanding the dynamics of CPF - 220:09
Understanding the dynamics of CPF - 320:29
Understanding the dynamics of CPF - 420:33
Understanding the dynamics of CPF - 520:36
How is the nuclease activated?21:45
CPF nuclease - 122:14
CPF nuclease - 223:38
X-ray crystal and cryoEM structure of Ysh1-Mpe1 complex24:13
The active site is located at the end of a long tunnel - 124:31
The active site is located at the end of a long tunnel - 224:53
Cryo-EM of Ysh1-Mpe1-Ipa1 complex25:10
Reconstitution of cleavage activity by the Ysh1 nuclease - 125:46
Reconstitution of cleavage activity by the Ysh1 nuclease - 226:47
Reconstitution of cleavage activity - 127:36
Reconstitution of cleavage activity - 228:09
Negative Stain EM of Core CPF29:13
Architecture of core CPF - 129:45
Architecture of core CPF - 230:01
Architecture of core CPF - 331:08
Mpe1 binds the polymerase module - 132:03
Mpe1 binds the polymerase module - 232:23
Mpe1 binds the polymerase module - 332:31
Mpe1 is important for cleavage35:03
Mpe1 is important for cleavage and polyadenylation - 136:28
Mpe1 is important for cleavage and polyadenylation - 237:01
Mpe1 is important for cleavage and polyadenylation - 337:23
Mpe1 is important for cleavage and polyadenylation - 438:55
Dynamics likely regulate CPF activation - 139:09
Dynamics likely regulate CPF activation - 239:31
Dynamics likely regulate CPF activation - 339:45
Dynamics likely regulate CPF activation - 439:46
What about the human complex?40:46
RBBP6/Mpe1 is not a core subunit of human CPSF - 141:46
RBBP6/Mpe1 is not a core subunit of human CPSF - 242:08
RBBP6/Mpe1 is not a core subunit of human CPSF - 342:23
RBBP6 activates human pre-mRNA cleavage - 143:17
RBBP6 activates human pre-mRNA cleavage - 243:42
RBBP6 activates human pre-mRNA cleavage - 344:02
RBBP6 activates human pre-mRNA cleavage - 444:11
RBBP6 activates human pre-mRNA cleavage - 544:54
RBBP6 activates human pre-mRNA cleavage - 645:09
RBBP6 activates human pre-mRNA cleavage - 745:32
Reconstitution of 3ʹ-end processing - 145:44
Reconstitution of 3ʹ-end processing - 245:50
MRC46:15