0.25
0.5
0.75
1.25
1.5
1.75
2
Harnessing nanopores for single-molecule enzymology and protein sequencing
Published on Sep 25, 2018410 Views
Biological nanopores are a class of membrane proteins that form nanometer-size apertures on lipid membranes. Under an applied potential the ionic current through nanopores is used to identify molecule
Related categories
Chapter list
Harnessing nanopores for single-molecule enzymology and protein sequencing00:00
Outline01:18
Protein transport across nanopores02:44
Biological nanopore04:31
Biological nanopore technology06:03
Nanopore technology06:49
Trapping Forces : Electrophoresis08:09
Electroosmotic flow09:54
Single-molecule DNA sequencing13:01
Protein analysis with nanopore: challenges14:35
Applications: protein sequencing16:28
Protein Mapping17:50
Identification of Folded Proteins19:12
Single-Molecule enzymology20:39
This talk23:47
Nanopores - 124:19
Nanopores - 224:35
Applications25:17
A nanopore test-tube25:45
Protein entry26:31
Proteins inside the nanopore27:15
SBD proteins28:20
Capture29:22
SBD29:39
SBD - 230:44
FRET Studies30:59
Confinement33:00
Why33:42
Ohther SBD protein tested:33:54
SBD234:01
explanation34:07
Orientation34:21
SBD2 tumbles inside the pore34:50
Detection from bodily fluids - 136:46
Detection from bodily fluids - 237:01
Peptide analysis and sequencing - 137:04
Peptide analysis and sequencing - 237:48
Biomarker analysis38:07
Protein Capture - 138:45
Protein Capture - 238:59
Peptide Capture - 339:26
Peptide Capture - 439:37
Why?39:50
Magic pH 4.541:01
β2-Microglobulin41:43
EGF human Endothelin 142:43
Angiotensin I42:51
Polypeptide recognition43:03
Conclusion44:19
Aknoweledgments46:06