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Mass spectrometry for oligonucleotide analysis: from sequencing to biophysical characterization
Mass spectrometry became a versatile tool for small molecule characterization and for protein sequencing, but maybe less well known is its potential use for biophysics. The mass of the complex gives i
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Presentation
Mass Spectrometry for Oligonucleotide Analysis: from Sequencing to Biophysical Characterization00:00
What is mass spectrometry research?00:47
What is mass spectrometry research?01:06
Molecular Systems01:49
“Native Mass Spectrometry”02:23
Electrospray: from Solution to the Gas Phase03:10
Electrospray: from Solution to the Gas Phase03:47
Reading a mass spectrum: assumption-free stoichiometry determination!04:31
Expected G-quadruplex (G4) Stoichiometries06:29
MS can reveal unexpected G4 stoichiometries07:24
Current research: meaning of charge state distributions09:59
What is mass spectrometry research?10:56
Determination of binding constants by native MS12:13
Determination of binding constants by native MS12:48
Determination of binding constants by native MS13:21
High [NH4OAc] => minimal differences in ESI response14:17
Electrospray MS screening of ligand-target interactions15:45
Electrospray MS screening of ligand-target interactions17:52
Revealing G-quadruplex folding pathways19:12
Revealing G-quadruplex folding pathways20:06
Revealing G-quadruplex folding pathways22:30
Revealing G-quadruplex folding pathways24:09
Revealing G-quadruplex folding pathways25:07
Revealing G-quadruplex folding pathways25:50
Revealing G-quadruplex folding pathways27:12
Native MS studies of ligand binding modes27:36
Native MS studies of ligand binding modes29:06
Take-home messages29:16
What is mass spectrometry research?31:40
What is mass spectrometry research?32:00
There are different ways to fragment32:04
There are different ways to fragment32:31
Top-down sequencing with MS/MS33:07
Top-down sequencing with MS/MS35:05
Noncovalent ligand binding site mapping36:02
Noncovalent ligand binding site mapping36:47
Characterizing 3D structure with fragmentation?39:16
What is mass spectrometry research?39:50
What is mass spectrometry research?39:55
Determining Ion Shape with Ion Mobility MS40:11
Ion mobility interpretation requires understanding rearrangements between solution and gas-phase41:26
Ion mobility interpretation requires understanding rearrangements between solution and gas-phase42:22
IntramolecularG-quadruplexes: extra base pairs reducethe multiplicityof rearrangementpathways43:39
Current work: charge dependence of CCS45:30
Coulomb energy difference between states46:23
Take-home messages47:20
Acknowledgements48:26
Thank you48:52