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Single-particle cryo-EM: Visualization of biological molecules in their native states

Published on Oct 14, 201937 Views

The aim of Structural Biology is to explain life processes in terms of macromolecular interactions in the cell. These interactions typically involve more than two partners, and can run up to dozens.

Preglov kolokvij

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Chemistry

Chapter list

Single-particle Cryo-EM -- Visualization of Biological Molecules in their Native States - 100:00
Single-particle Cryo-EM -- Visualization of Biological Molecules in their Native States - 301:10
Single-particle Cryo-EM -- Visualization of Biological Molecules in their Native States - 202:02
Molecular Machines in the Cell - 102:35
MULTIPLE STATES OF A MOLECULAR MACHINE: THE RIBOSOME05:30
Molecular Machines in the Cell - 206:44
X-ray Crystallography07:40
Electron Microscopy11:01
THREE-DIMENSIONAL RECONSTRUCTION: STRUCTURES WITH HELICAL SYMMETERY - 113:52
THREE-DIMENSIONAL RECONSTRUCTION: VIRUSES WITH ICOSAHEDRAL SYMMETRY - 215:55
THREE-DIMENSIONAL RECONSTRUCTION: VIRUSES WITH ICOSAHEDRAL SYMMETRY - 316:38
WHY CRSTALS? 3D Reconstruction of Asymmetrical Molecules by Electron Tomography - 117:36
WHY CRSTALS? 3D Reconstruction of Asymmetrical Molecules by Electron Tomography - 219:15
EM images can be aligned to within better than 3 Angstrom! - 120:41
EM images can be aligned to within better than 3 Angstrom! - 221:24
SHORT NOTE21:54
CONDITIONS FOR ALIGNMENT OF TWO IMAGES OF A MOLECULE OF SIZE D23:08
Devil in the detail – Problems to be solved:24:58
SPIDER -- Modular image processing program27:02
Alignment and averaging29:26
Problem of heterogeneity: molecules are in different orientations and conformations30:09
Multivariate analysis of aligned molecule images31:02
RANDOM-CONICAL RECONSTRUCTION – PRINCIPLE - 131:34
RANDOM-CONICAL RECONSTRUCTION – PRINCIPLE - 232:40
RANDOM-CONICAL RECONSTRUCTION – PRINCIPLE - 334:13
RANDOM-CONICAL RECONSTRUCTION – PRINCIPLE - 434:35
RECONSTRUCTION OF 50S RIBOSOMAL SUBUNIT FROM E. COLI RIBOSOME - 135:43
RECONSTRUCTION OF 50S RIBOSOMAL SUBUNIT FROM E. COLI RIBOSOME - 235:46
Frozen-hydrated specimen / freeze-plunging / vitreous ice / cryo-EM - 137:27
Frozen-hydrated specimen / freeze-plunging / vitreous ice / cryo-EM - 238:57
Plunge-Freezer - 139:39
Plunge-Freezer - 240:21
Plunge-Freezer - 340:55
Plunge-Freezer - 441:49
Elongation Cycle (for adding each amino acid)43:58
DISCOVERY OF RATCHET-LIKE MOVEMENT DURING TRANSLOCATION44:33
MAXIMUM LIKELIHOOD METHODS OF CLASSIFICATION45:19
“STORY IN A SAMPLE” -- intermediate states in the ratchet-like motion and hybrid tRNA positions in the absence of EF-G46:09
MILESTONES IN SINGLE-PARTICLE RECONSTRUCTION - 247:14
New era (since 2012): New single-electron detecting cameras - 147:40
New era (since 2012): New single-electron detecting cameras - 248:27
Elongation Factor G mutant H94A bound to the ribosome48:41
T. cruzi ribosome large subunit at 2.5 Å - 149:04
T. cruzi ribosome large subunit at 2.5 Å - 249:45
T. cruzi ribosome large subunit at 2.5 Å - 350:19
Ryanodine Receptor50:49
CYSTIC FIBROSIS51:22
Open and Closed states of mutant 508del-hCFTR - 151:29
Conclusion -- Single-particle cryo-EM: A new era in structural biology51:47
Impact in Biology and Molecular Medicine - 153:26
Impact in Biology and Molecular Medicine - 254:11