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Activities of GMO analysis in China
Published on Dec 31, 20151663 Views
As the rapid research and application of transgenic techniques in modern agriculture, more and more new GM crops were developed and commercialized in China. Since the GMO labelling regulation was issu
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Chapter list
Activities of GMO analysis in China00:00
SJTU History00:35
Campus01:01
History01:40
Zhang lab major research Interests02:14
Biosafety and Food Safety02:41
Outline - 103:23
Chinese commercialized GM plants03:33
GM plants (Peking University)04:04
GM papaya04:24
GM cotton for insect resistance04:44
GM rice and GM maize got the certificates since 200805:03
GM fish06:32
Transgenic cloning cattle and goat07:16
Outline - 207:37
Legislation systems07:57
A total of 17 kinds of GM products should be labeled08:16
Most of the oil productions were labeled with GM materials08:59
Detection labs of GMOs in China09:09
Currently used techniques in routine analysis10:04
Development, validation, and harmonization of Endogenous reference genes - 111:13
Development, validation, and harmonization of Endogenous reference genes - 211:48
One crop, one endogenous reference gene12:23
The suitable endogenous reference genes for rice 14:05
Development and validation of PCR and quantitative real-time PCR methods14:09
A Multiplex Degenerate PCR Analytical Approach14:56
Degenerate PCR assays for bar/pat and cp4-epsps have been adapted in national standards15:52
Standardization of PCR and quantitative real-time PCR methods16:13
The standard systems of GMO analysis16:47
Development of lateral flow device for field test17:45
New methods for quick, high thoughput analysis18:12
An Integrative System Combining a rapid DNA Extraction21:32
The procedure of detect GMO products based on the nucleic acids21:45
Diagram of the developed DNA extraction device22:00
Agarose gel electrophoresis analysis22:38
Sensitivity of event-specific assays using Genomic DNA23:07
Field test employing GM rice as examples24:02
A high-throughput Analytical Method for Multiple DNA Targets24:58
Droplet PCR (W/O emulsion PCR)25:07
Multiplex Microdroplet PCR Implemented CGE26:00
Optimization of the MPIC System27:06
The Flexibility of the MPIC Setup27:41
A High-throughput and Flexible Platform for Monitoring of Genetically Modified Organisms28:18
Principles28:26
Monitoring results of the simulated samples30:03
The coverage of the MACRO platform for known GMOs30:23
One-step Visual Detection of Multiple Nucleic Acids in a Microcapillary Array30:59
Basic principle and worflow31:11
Sensitivity evaluation should this array has high sensitivity in different GM samples testing32:40
The accurate results of the practical sample tests33:03
Outline - 333:40
Four types CRMs will be developed for GMO analysis33:50
Establishment of the technical systems for matrix-based CRMs34:27
Identification of the candidate materials - 135:04
Identification of the candidate materials - 235:22
Homogeneity study - Stability study35:49
Values of the GM rice RMs expressed with copies ratio36:01
Establishment of the technical systems for plamid DNA CRMs36:43
Strategy for GM plasmid RM construction37:20
Constructed several plasmid RMs for GM maize, rice and cotton events37:35
Schematic diagram of plasmid pSOY and pCanola37:52
Specificity evaluation - Sensitivity evaluation38:17
Update, the plasmid RMs for 48 GM events were developed38:26
Summary38:57
Family Album40:02
Thanks for your attention40:25