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Pseudomonas syringae pv. actinidiae, three years after its discovery in New Zealand: what have we learned about the pathogen, the disease and how to control it?
Published on 2013-10-143949 Views
In the last few years the causal agent of bacterial canker of kiwifruit, Pseudomonas syringae pv. actinidiae (Psa), has become a global pathogen of economic importance. Before 2008, this little known
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Presentation
Pseudomonas syringae pv. actinidiae, tri leta po odkritju v Novi Zelandiji: kaj smo se naučili o patogenu, o bolezni in kako se jo obvladuje00:00
New Zealand00:33
The New Zealand kiwifruit industry in perspective ...01:05
Early November 2010: Few leaf spots ...02:17
Earlier in Italy ...02:42
The follow up in Italy (2009)02:50
Identification of Pseudomonas syringae pv actinidiae by PCR using the primers PsaF1/R203:39
November 2010 detection of Psa in New Zealand04:46
Box PCR patterns and DNA sequence of cts gene05:53
Box PCR patterns of Psa strains from New Zealand06:51
Box PCR patterns and pathogenicity of Psa strains from New Zealand07:21
Box PCR patterns and cts haplotypes from Psa strains from New Zealand07:47
Difference in virulence between biovar 3 and biovar 4 of Psa08:02
Bayesian inference phylogenetic tree of Pseudomonas syringae pv. actinidiae - 108:25
Effector genes in strains of P. syringae pv. actinidiae from different geographic origins09:14
Bayesian inference phylogenetic tree of Pseudomonas syringae pv. actinidiae - 209:42
Similar grouping by MLST using house keeping genes or effector genes09:54
Phylogeny of Psa based on Whole Genome Sequence10:45
November 2010 detection of biovar 3 Psa in New Zealand12:46
Bay of Plenty December 201013:10
What is the situation today? (18 September 2013)14:43
New Zealand kiwifruit growing regions15:38
Cost of Psa in New Zealand (2012 study)16:13
Cost of Psa in New Zealand (2013 estimate)17:28
Climatic considerations19:19
Plant 1A: ‘Hort16A’ 3 weeks after inoculation20:23
Plant 1B: ‘Hort16A’ 3 weeks after inoculation21:09
Psa populations in cfu/g - 121:48
Plant 1A: Constant humidity - 122:18
Plant 1A: Constant humidity - 222:45
Plant 1A: Constant humidity - 322:49
Psa populations in cfu/g - 222:52
Plant 1B: 48 hours high humidity24:04
Other contributing factors to the intensity of the New Zealand outbreak24:10
Epidemiology: where does Psa survive? - 125:00
Epidemiology: where does Psa survive? - 225:15
Survival on leaf litter (25 May – 1 September)25:36
Survival of Psa on symptomatic cane material Tyson et26:32
Unique and shared ortholog groups between biovars26:48
Effector complement varies considerable between Psa biovars27:34
Psa V-13 chromosome (biovar 3)28:31
ICEs are the key difference between biovar 3 isolates28:51
Distribution of Integrative Conjugative Elements in isolates from China30:00
Inoculation of lower leaf surface31:23
Colonisation of the stomata and bacteria exuding from stomata31:55
Leaf infection31:59
Infected cane (lenticel)32:38
Psa infected cane cross-section 1000x - 133:25
Psa infected cane cross-section 1000x - 234:04
Psa infected cane cross-section 1000x - 334:21
Colonisation of the xylem by Psa34:26
Coronatine structural and functional homologue of jasmonic acid35:08
Incidence of Psa on ‘Bruno’ seedlings treated with Methyl-Jasmonate 7 days before inoculation35:56
Signalling pathways in Arabidopsis37:05
Bruno’ seedlings treated with SA or ASM 7 days before inoculation37:53
Field experiments39:08
Disease incidence 21 days after inoculation with Psa39:58
In conclusion42:00
Untitled44:04