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Pseudomonas syringae pv. actinidiae, three years after its discovery in New Zealand: what have we learned about the pathogen, the disease and how to control it?
Published on Oct 14, 20133941 Views
In the last few years the causal agent of bacterial canker of kiwifruit, Pseudomonas syringae pv. actinidiae (Psa), has become a global pathogen of economic importance. Before 2008, this little known
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Pseudomonas syringae pv. actinidiae, tri leta po odkritju v Novi Zelandiji: kaj smo se naučili o patogenu, o bolezni in kako se jo obvladuje00:00
New Zealand00:33
The New Zealand kiwifruit industry in perspective ...01:05
Early November 2010: Few leaf spots ...02:17
Earlier in Italy ...02:42
The follow up in Italy (2009)02:50
Identification of Pseudomonas syringae pv actinidiae by PCR using the primers PsaF1/R203:39
November 2010 detection of Psa in New Zealand04:46
Box PCR patterns and DNA sequence of cts gene05:53
Box PCR patterns of Psa strains from New Zealand06:51
Box PCR patterns and pathogenicity of Psa strains from New Zealand07:21
Box PCR patterns and cts haplotypes from Psa strains from New Zealand07:47
Difference in virulence between biovar 3 and biovar 4 of Psa08:02
Bayesian inference phylogenetic tree of Pseudomonas syringae pv. actinidiae - 108:25
Effector genes in strains of P. syringae pv. actinidiae from different geographic origins09:14
Bayesian inference phylogenetic tree of Pseudomonas syringae pv. actinidiae - 209:42
Similar grouping by MLST using house keeping genes or effector genes09:54
Phylogeny of Psa based on Whole Genome Sequence10:45
November 2010 detection of biovar 3 Psa in New Zealand12:46
Bay of Plenty December 201013:10
What is the situation today? (18 September 2013)14:43
New Zealand kiwifruit growing regions15:38
Cost of Psa in New Zealand (2012 study)16:13
Cost of Psa in New Zealand (2013 estimate)17:28
Climatic considerations19:19
Plant 1A: ‘Hort16A’ 3 weeks after inoculation20:23
Plant 1B: ‘Hort16A’ 3 weeks after inoculation21:09
Psa populations in cfu/g - 121:48
Plant 1A: Constant humidity - 122:18
Plant 1A: Constant humidity - 222:45
Plant 1A: Constant humidity - 322:49
Psa populations in cfu/g - 222:52
Plant 1B: 48 hours high humidity24:04
Other contributing factors to the intensity of the New Zealand outbreak24:10
Epidemiology: where does Psa survive? - 125:00
Epidemiology: where does Psa survive? - 225:15
Survival on leaf litter (25 May – 1 September)25:36
Survival of Psa on symptomatic cane material Tyson et26:32
Unique and shared ortholog groups between biovars26:48
Effector complement varies considerable between Psa biovars27:34
Psa V-13 chromosome (biovar 3)28:31
ICEs are the key difference between biovar 3 isolates28:51
Distribution of Integrative Conjugative Elements in isolates from China30:00
Inoculation of lower leaf surface31:23
Colonisation of the stomata and bacteria exuding from stomata31:55
Leaf infection31:59
Infected cane (lenticel)32:38
Psa infected cane cross-section 1000x - 133:25
Psa infected cane cross-section 1000x - 234:04
Psa infected cane cross-section 1000x - 334:21
Colonisation of the xylem by Psa34:26
Phylogeny of Psa based on Whole Genome Sequence34:42
Coronatine structural and functional homologue of jasmonic acid35:08
Signalling pathways in Arabidopsis35:19
Incidence of Psa on ‘Bruno’ seedlings treated with Methyl-Jasmonate 7 days before inoculation35:56
Signalling pathways in Arabidopsis37:05
Bruno’ seedlings treated with SA or ASM 7 days before inoculation37:53
Field experiments39:08
Disease incidence 21 days after inoculation with Psa39:58
In conclusion42:00
Untitled44:04