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Brain repair after stroke – applying stem cells, biomaterials and in vivo molecular imaging in mouse within the GlowBrain platform
Published on Jul 24, 20151754 Views
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Chapter list
Brain repair after stroke – using the GlowBrain platform to combine stem cells, biomaterials, and in vivo molecular imaging in mouse00:00
Contribution to the substantial change in medicine02:47
Combining Stem Cells and Biomaterials for Brain Repair04:08
REGPOT GlowBrain -> Regenerative medicine04:32
GlowBrain three main pillars04:41
Mouse model for the human stroke05:12
In vivo imaging modalities (multimodal imaging)06:38
Small animal MRI07:47
Nanoparticles for MRI imaging - 109:02
Nanoparticles for MRI imaging - 209:49
Nanoparticles for MRI imaging - 310:11
Nanoparticles for MRI imaging - 410:40
Nanoparticles for MRI imaging - 510:58
Nanoparticles for MRI imaging - 611:00
In vivo imaging system (IVIS)11:11
Bioluminescent imaging (BLI)11:38
Post-ischemic Inflammation - 113:25
Post-ischemic Inflammation - 214:08
Tlr2/luc/gfp transgenic mouse model for bioluminescent imaging (BLI)14:40
TLR2 expression analysis after ischemic brain injury15:00
TLR2 in vivo expression analysis - 115:04
TLR2 in vivo expression analysis - 216:09
TLR2 in vivo expression analysis - 316:23
TLR2-/- vs. wild type comparison16:28
Medial cerebral artery occlusion (MCAO)16:37
Inflammation in TLR2-/- mice? - 116:47
Inflammation in TLR2-/- mice? - 216:51
Inflammation in TLR2-/- mice? - 316:58
Results - 117:12
Results - 217:19
TLR2-/- consequences - 117:21
TLR2-/- consequences - 217:30
TLR2-/- consequences - 318:02
Mechanism? - 119:46
Mechanism? - 219:48
Mechanism? - 320:22
Mechanism? - 420:29
Mechanism? - 520:30
Mechanism? - 620:31
Mechanism? - 720:35
TLR2 function is time dependent!20:53
Time dependent postishemic brain response!21:23
GAP-43 and ischemic brain injury22:12
GAP-4322:22
VivoGloTM Caspase 3/7 substrate – ZDEVD-Aminoluciferin - 122:49
VivoGloTM Caspase 3/7 substrate – ZDEVD-Aminoluciferin - 223:08
In vivo imaging of Caspase 3/7 and GAP43 coexpression using VivoGlo™ prosubstrate23:49
Cells that produce bioluminiscent signal in GAP- 43/luc/gfp mice are GAP-43 positive neurons24:05
GAP-43 is in the apoptotic cells24:12
GAP-43 expression in cells undergoing stress - 124:25
GAP-43 expression in cells undergoing stress - 224:37
GAP-43 is a candidate to be marker of neruonal stress in the early phase of brain injury24:40
2 mouse in vivo imaging models for 3 purposes24:50
Alginate25:06
Alginate gelation and cell encapsulation25:12
TLR2-luc as a model to test in vivo biocompatibility of transplanted cells and/or biomaterials27:00
Alginate Biocompatibility27:57
Alginate is biocompatibile for brain use28:02
Injecting mesenchymal stem cells after MCAO-induced stroke28:09
Tlr2-luc bioimaging - 128:24
Tlr2-luc bioimaging - 229:05
TLR2-luc can be used for monitor stem cell effects in the brain29:13
Acknowledgments29:19