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Plasmids and virulence in E. amylovora and related pathogens
Published on May 25, 20123408 Views
Erwinia amylovora is the quarantine organism responsible for the fire blight of rcsacecus plants. The disease can affect pear tree, apple and loquat, and to diverse ornamental plants. This bacterium a
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Plasmids and virulence in E. amylovora and related pathogens00:00
IVIA - Instituto Valenciano de Investigaciones agrarias00:42
Lab. of Bacteriology. Plant Protection and Biotechnology Center01:27
Plasmids and virulence in E. amylovora and related pathogens01:51
The pathogen02:27
Symptoms of fire blight - 103:47
Symptoms of fire blight - 204:08
Plasmids in Erwinia amylovora04:18
Characteristics of Pea29 plasmid05:19
Molecular genetics involved in virulence and pathogenicity in Erwinia amylovora06:31
Fire blight in Spain (1995-2011)07:06
Characterization of spanish strains08:49
PCM with primers from plasmid pEA29 and chromosome09:41
Plasmids of strain IVIA 1614-2a and reference strainsa10:19
Analysis of plasmids by restriction digestion with BamHI11:15
Approximate size of plasmid from strain IVIA 1614-2a after restriction with BamHI11:40
Hybridization analyses of the plasmids11:48
Hybridization analyses after restriction with enzyme EcoRI12:12
Hybridization of total DNA of strain IVIA 1614-2a with pEA29 probe12:35
Characteristics of strain IVIA 1614-2a12:46
Growth curve of reference strains and IVIA 1614-2a13:36
Virulence studies: inoculation in several hosts13:55
Virulence in plants and immature pear: comparison with reference strains14:15
Study of virulence in natural E. amylovora strains devoid of the pEA29 kb plasmid - 114:35
Analyses performed15:48
Plasmid profile of the isolates obtained in the outbreak in segovia16:15
Plasmid profiles of strains without pEA29 isolated in nature16:59
Study of virulence in natural E. amylovora strains devoid of the pEA29 kb plasmid - 217:25
Labelling and curing of pEI7017:44
Virulence assays: inoculation in immature pear18:39
Effect of plasmids pEA29 and pEI70 on virulence18:56
Virulence of strains with mutants pEI70::Tn5 kanR introduced inoculation in immature pear20:45
Inoculation mutants pEI70::Tn5 kanR in immature pear21:01
Role of pEI70 in different strains with reduced virulence21:14
Remarks21:40
Presence of pEI70 in spanish and european isolates22:28
Duplex analyses of strains from european countries23:07
Distribution of strains with pEI70 in Europe23:45
Analyses of strains from other continents24:03
Related pathogenic Erwinia described and plasmids24:39
Genetic comparison of plasmid pEI70 with related bacteria26:08
Characteristics of the pEI70 plasmid27:10
Plasmids in pathogenic fruit tree Erwinia species: do they have a common function and origin?27:57
Plasmid content of related Erwinias29:12
Hybridization analyses with related Erwinias29:19
Restriction analysis with BamHI29:30
Hybridization analyses with pEA2929:32
Comparison29:39
Regions of pEPIR37 not detected in plasmids of other pahogenic Erwinia30:24
Effect of pEPIR37 in E. amylovora30:56
Inoculation analyses in immature loquat31:47
Geographical origin of different Erwinias31:59
Conclusions32:33
Thank you33:34