0.25
0.5
0.75
1.25
1.5
1.75
2
Lizosomalni katepsini in njihovi endogeni inhibitorji − 50 let raziskav
Published on Apr 23, 20152410 Views
Odkritje celične organele lizosoma leta 1955 kot glavne komponente endozomalno-lizosomalnega sistema predstavlja prelomnico v razumevanju procesov intracelularne razgradnje proteinov. V zelo kratkem č
Related categories
Chapter list
Lizosomalni katepsini in njihovi endogeni inhibitorji − 50 let raziskav00:00
Historical Milestones05:56
Lysosomes as biochemical concept (rat liver lysosomes, 1963)* - 110:32
Lysosomes as biochemical concept (rat liver lysosomes, 1963)* - 212:22
Hans Neurath(1909-2002)13:36
Current numbers of peptidases in humans and other species15:59
Cathepsins16:24
Papain-like (cysteine) cathepsins - 118:15
Papain-like (cysteine) cathepsins - 219:29
Papain-like (cysteine) cathepsins - 319:34
Papain-like (cysteine) cathepsins - 419:50
Papain-like (cysteine) cathepsins - 519:57
Substrate binding sites20:06
Physiological and pathophysiological role of cysteine cathepsins and their protein inhibitors21:47
Genetic diseases involving cysteine cathepsins and their protein inhibitors22:56
Propeptides of papain-like cysteine proteases24:12
Sequencealignment of human cysteine cathepsins24:41
Cathepsin B25:28
Cathepsin H26:03
Cathepsin C (DPPI)26:29
Cathepsin C (DPPI)tetramer29:11
Mutations of DPPI resulting in genetic diseases29:45
Y323 C mutation of DPPI30:43
Keratosis Palmoplantaris31:22
Cathepsins X, H, Band Csuperimposed on papainsurface31:44
Principles of protease activity regulation31:47
Inhibitors of papain-like cysteine peptidases31:50
Alignment of stefins, cystatins and kininogens33:37
The 2.0 A X-ray crystal structure of chicken egg white cystatin and its possible mode of interaction with cysteine proteinases36:15
Human Stefin B: polypeptide fold37:21
The refined 2.4 A X-ray crystal structure of recombinant human stefin B in complex with thecysteine proteinase papain: a novel type of proteinase inhibitor interaction.38:11
Magazine cover38:56
Cathepsin L-p41 fragment complex39:17
Actinia equina41:33
Thyropins: Schematic diagram of Tg type-1 repeats42:02
When a protease is druggable by a small compound?42:43
Protein structure based drug design cycle45:38
Steps in drug discovery •47:42
Small molecule inhibitor design48:20
Epoxy-succinyl inhibitors48:57
Cathepsin B complexed with epoxysuccinyl based inhibitor (CA030)49:55
Lessons from unsuccessful experiments:50:11
Pathophysiological function •51:06
Cystatin C fold Sanders et51:33
How to address biological functions of cathepsins?53:20
Lysosomal cathepsins and cancer54:06
Bid cleavage by lysosomal proteases55:06
Improvedanti-tumour effect of JPM-565inhibitor - 155:48
Improvedanti-tumour effect of JPM-565inhibitor - 256:03
What is protease signaling?56:33
Challenges in protease signaling56:34
Identification of protease substrates56:39
Cleavage site specificity for cathepsins K, L and S56:48
Perspectives in the field57:11
Statistics republic of Slovenia ( Nov. 7, 2014 )01:01:28
UNESCO ( statistics 2014): %BDP v R&D01:02:02
Our future01:02:54