0.25
0.5
0.75
1.25
1.5
1.75
2
Dizajn molekulskega origamija za nove molekulske stroje in uravnavanje delovanja celic
Published on Jun 04, 2018990 Views
Proteini so najbolj kompleksne molekule v naravi, ki hkrati predstavljajo »pametne nanostrukture«, saj linearno zaporedje aminokislin določa njihovo strukturo in funkcijo. Kljub zelo kompleksnemu pris
Related categories
Chapter list
Dizajn molekulskega origamija za nove molekulske stroje in uravnavanje delovanja celic00:00
Cellular factories and natural molecular machines03:34
Modular building blocks in machine engineering04:22
Natural biopolymers with designable structure05:07
Natural protein origami06:15
Designed protein domain assemblies07:42
Modular shapes08:42
DNA nanotechnology09:12
Natural and designed bionanostructures10:20
Dimeric building modules11:07
Coiled-coils as building blocks13:03
Flexible linker connecting interacting elements - 114:57
Flexible linker connecting interacting elements - 215:16
Deconstructing shape into modules - 115:27
Deconstructing shape into modules - 215:54
Topological analysis of paths16:23
Topological solutions for a tetrahedron16:54
Design of a tetrahedral protein cage17:38
TEM and AFM imaging19:04
Termini of the tetrahedral path coincide19:27
Coincidence of termini by FP reconstitution20:23
Natural and coiled-coil protein origami fold20:58
Polyhedra from parallel and antiparallel edges21:59
Protein folding pathway23:04
Kinetic determination of Topological knots23:39
DNA as the prototyping material25:08
Selection of the folding pathway based on the DNA twist25:54
Relations of the two polynucleotide loops26:40
Allowed and disallowed 3-loop topologies26:52
Building modules of different stability27:36
Square pyramid designs from the same segments27:55
oxDNA coarse grained simulation29:06
Simulation of the kinetics of folding steps29:31
Bottleneck steps in folding30:27
Rapid quenching of DNA designs30:52
Isothermal self-assembly of DNA designs32:15
Pipeline for the design of protein polyhedra32:55
Tunable stability of building modules34:27
Supercharging of CC dimer modules36:08
Orthogonality of supercharged CC modules36:23
Production of the second generation protein origami without refolding36:43
Second generation protein origami37:14
Crosslinking analysis of the topology of TET12SN37:36
SAXS analysis of TET12SN38:01
Polypeptide tetrahedra with different topology38:39
Protein variants to establish the design rules38:56
Rectangular pyramid39:28
Trigonal prism39:45
Structural analysis40:08
In vivo production and biocompatibility40:38
Expression of protein origami in vivo is harmless41:28
Protein interaction domains41:55
Dimerization specificity of CC peptides in human cells42:41
Orthogonality of the designed CC peptide set43:07
CC peptides for directing protein localization43:43
Strategies for the enhanced transcriptional activation44:32
Fine-tuning of transcriptional strength45:00
Enhanced transcription by CC concatenation45:26
Challenges for the future46:00
Acknowledgements47:26
Hvala lepa !48:08
De novo design of orthogonal coiled-coil pairs56:40