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Through different eyes... Forty years of nuclear microscopy at the micrometre scale
Published on Jan 15, 202064 Views
2020 marks the fortieth anniversary of the first published images using ion beam analysis (IBA) at a spatial resolution of 1 micrometre. IBA exploits the reaction products emitted when samples are ex
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Chapter list
Through different eyes. Forty years of nuclear microscopy at the micrometre scale00:00
Outline00:22
Ion Beam Analysis (IBA)01:26
A closer look at PIXE02:54
Tracks of MeV ions in matter05:37
Nuclear Microscopy06:14
Two ways to make a microbeam:07:13
Lenses for MeV ions08:33
Regimes of microbeam instruments09:33
1970: The Harwell Microprobe (UK)11:50
the first published Nuclear Microbeam image12:06
1980: Oxford12:49
1980: The first Oxford proton microbeam14:19
Some early result from Oxford in the 1980s15:11
1988: “Monolithic” lenses (Oxford)16:15
2002 to today… University of Surrey Ion Beam Centre17:28
Surrey nanobeam line18:48
PIXE results from the Surrey nanobeam18:56
External beam / MeV-SIMS20:22
CIBA20:29
Current state of the art resolutions 21:21
Ways of using MeV ion Microbeams21:56
MicroPIXE is IDEAL for investigating the role of metals in biological systems23:42
Metals in insect exoskeletons - 124:33
Metals in insect exoskeletons - 226:02
Metals in insect exoskeletons - 327:18
Identifying and quantifying metal atoms in protein molecules27:41
Metals in proteins28:50
PIXE analysis of metals in proteins - 130:39
Dependence on sample thickness33:00
PIXE analysis of metals in proteins - 233:52
For example …34:32
High throughput analysis35:25
Sample preparation36:32
Automated array analysis37:14
The Protein Data Bank (PDB)37:47
Samples38:59
Results40:04
Why the discrepancies?41:00
Processing X-ray diffraction data42:05
3DCP - 143:14
3DCP - 244:10
3DCP - 344:53
Conclusion45:04
Elemental mapping in air:46:09
The Rosslyn Chapel46:14
The windows46:30
Weathered panel47:09
Analysis - 147:15
Analysis - 248:02
“Raw” elemental maps (front face) - 148:48
“Raw” elemental maps (front face) - 249:20
Processed images49:22
Through different eyes50:15
Thank you51:01