0.25
0.5
0.75
1.25
1.5
1.75
2
Through different eyes... Forty years of nuclear microscopy at the micrometre scale
Published on 2020-01-1580 Views
2020 marks the fortieth anniversary of the first published images using ion beam analysis (IBA) at a spatial resolution of 1 micrometre. IBA exploits the reaction products emitted when samples are ex
Related categories
Presentation
Through different eyes. Forty years of nuclear microscopy at the micrometre scale00:00
Outline00:22
Ion Beam Analysis (IBA)01:26
A closer look at PIXE02:54
Tracks of MeV ions in matter05:37
Nuclear Microscopy06:14
Two ways to make a microbeam:07:13
Lenses for MeV ions08:33
Regimes of microbeam instruments09:33
1970: The Harwell Microprobe (UK)11:50
the first published Nuclear Microbeam image12:06
1980: Oxford12:49
1980: The first Oxford proton microbeam14:19
Some early result from Oxford in the 1980s15:11
1988: “Monolithic” lenses (Oxford)16:15
2002 to today… University of Surrey Ion Beam Centre17:28
Surrey nanobeam line18:48
PIXE results from the Surrey nanobeam18:56
External beam / MeV-SIMS20:22
CIBA20:29
Current state of the art resolutions 21:21
Ways of using MeV ion Microbeams21:56
MicroPIXE is IDEAL for investigating the role of metals in biological systems23:42
Metals in insect exoskeletons - 124:33
Metals in insect exoskeletons - 226:02
Metals in insect exoskeletons - 327:18
Identifying and quantifying metal atoms in protein molecules27:41
Metals in proteins28:50
PIXE analysis of metals in proteins - 130:39
Dependence on sample thickness33:00
PIXE analysis of metals in proteins - 233:52
For example …34:32
High throughput analysis35:25
Sample preparation36:32
Automated array analysis37:14
The Protein Data Bank (PDB)37:47
Samples38:59
Results40:04
Why the discrepancies?41:00
Processing X-ray diffraction data42:05
3DCP - 143:14
3DCP - 244:10
3DCP - 344:53
Conclusion45:04
Elemental mapping in air:46:09
The Rosslyn Chapel46:14
The windows46:30
Weathered panel47:09
Analysis - 147:15
Analysis - 248:02
“Raw” elemental maps (front face) - 148:48
“Raw” elemental maps (front face) - 249:20
Processed images49:22
Through different eyes50:15
Thank you51:01