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Super-resolution optical microscopy: New insights and remaining tasks
Published on Sep 14, 20161462 Views
Efficient functioning of the human body involves a multi-level synthesis emerging from collective brain activity to the immune system. Understanding the complex interactions of the molecular processes
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Chapter list
Advanced optical microscopy: Challenges and prospects00:00
Live Cell Microscopy: Observation of living cells: Non-Invasive03:00
Optical Far-Field Microscopy: Fluorescence03:36
Far-Field Fluorescence Microscopy: Success Story04:12
Far-Field Fluorescence Microscopy: Demands04:35
T-Cells: Microscopy Demands - 105:06
T-Cells: Microscopy Demands - 206:08
Optical Microscopy: Trade-Offs08:00
Optical Microscopy: Trade-Offs: Solutions09:06
Optical Microscopy: Trade-Offs: Solution - Actin Cytoskeleton10:20
STED Microscopy: Examples - Organelles10:41
Lipid Plasma Membrane Organization: Nanoscale12:02
Lipid Plasma Membrane Organization: Interactions on the Nanoscale: Nanodomains12:56
Lipid Plasma Membrane Organization: Fluorescence Recordings: Lipids14:08
Lipid Plasma Membrane Organization: Imaging of lipid analogs14:56
Lipid Plasma Membrane Dynamics: Measurement mode15:33
Fluorescence Correlation Spectroscopy (FCS): Measurement of Diffusion Dynamics16:24
Lipid Plasma Membrane Dynamics: Confocal FCS Recordings - 117:25
Lipid Plasma Membrane Dynamics: Confocal FCS Recordings - 218:10
Far-Field Microscopy: Surpassing the Resolution Limit: Turning ON/OFF - 118:26
Far-Field Microscopy: Surpassing the Resolution Limit: Turning ON/OFF - 219:31
Lipid Plasma Membrane Dynamics: Move to STED - 120:30
Lipid Plasma Membrane Dynamics: Move to STED - 220:36
STED Microscopy: Dynamical confinement of resolution21:29
Lipid Plasma Membrane Dynamics: STED-FCS: Measurement Principle21:56
Lipid Plasma Membrane Dynamics: STED-FCS: Diffusion modes - 122:26
Lipid Plasma Membrane Dynamics: STED-FCS: Diffusion modes - 222:47
Lipid Plasma Membrane Dynamics: STED-FCS: Diffusion modes - 323:07
Lipid Plasma Membrane Dynamics: STED-FCS: PE vs SM - 123:25
Lipid Plasma Membrane Dynamics: STED-FCS: PE vs SM - 224:06
STED Microscopy: Photobleaching/Phototoxicity - 124:33
STED Microscopy: Photobleaching/Phototoxicity - 226:57
STED Microscopy: Photobleaching/Phototoxicity - 327:43
STED Microscopy: Photobleaching/Phototoxicity - 427:47
STED Microscopy: Photobleaching/Phototoxicity - 527:52
Optimization of Fluorescence Signal: STED - D-Rex28:54
STED Microscopy: Ultimate limit: NV centers in diamond29:09
STED Microscopy: Single NV centers - ultimate photostability29:59
STED Microscopy: Ultimate limit: NV centers in diamond30:13
Fluorescence Nanoscopy: GSD imaging by optical dark state shelving - 131:20
Fluorescence Nanoscopy GSD imaging by optical dark state shelving - 231:57
Far-Field RESOLFT Nanoscopy: Reversibly Photoswitchable Fluorescent Proteins - 132:36
Far-Field RESOLFT Nanoscopy: Reversibly Photoswitchable Fluorescent Proteins - 233:49
Far-Field RESOLFT Nanoscopy: Reversibly Photoswitchable Fluorescent Proteins - 334:39
Far-Field RESOLFT Nanoscopy: Reversibly Photoswitchable Fluorescent Proteins - 435:30
Advanced Microscopy: Trade-offs: Complementary Techniques - 135:49
Advanced Microscopy: Trade-offs: Complementary Techniques - 236:21
Advanced Microscopy: Trade-offs: Complementary Techniques - 437:05
Advanced Microscopy: Trade-offs: Complementary Techniques - 537:18
Acknowledgement38:13