The establishment and characterization of iPSC derived 3D neurosphere cultures from a Kleefstra patient

Published on Jun 14, 20236 Views

Kleefstra Syndrome Scientific Conference 2023: “Moving towards the uptake and use of Artificial Intelligence (AI) in research and clinical work”, Ljubljana 2023

The establishment and characterization of iPSC derived 3D neurosphere cultures from a Kleefstra patient00:00

EHMT1 mutation identified in a Kleefstra syndrome patient00:22

Previously estabilished01:03

Experimental workflow01:34

Spheroid formation and growth over time02:08

Neurite outgrowth02:42

Proliferation and growth of NPCs in control and Kleefstra cell lines03:28

Proliferation and growth growth of 3D_TD56 in control and Kleefstra cell lines04:39

qPCR analysis reveals diffrences in neuronal subtypes of the KS 3D_TD56 spheroids05:11

ICC stainin gof the spheroid sections confirms the qPCR data on protein level - 106:11

ICC stainin gof the spheroid sections confirms the qPCR data on protein level - 206:45

Oxidative stress response - 107:00

Oxidative stress response - 207:54

Gene expression09:30

Summary10:55