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ATP dependency of osmotically activated outwardly rectified current in the membrane of cytoplasmic droplets obtained from sporangiophore of model filamentous fungus Phycomyces blakesleeanus
Published on Jul 09, 2018377 Views
ORIC is outwardly rectified, rapidly inactivating anionic current that is activated in hypo-osmotic conditions in the membrane of cytoplasmic droplets obtained from sporangiophore of filamentous fungu
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ATP dependency of osmotically activated outwardly rectified current in the membrane of cytoplasmic droplets obtained from sporangiophore of model filamentous fungus Phycomyces blakesleeanus00:00
Only 6 cell membrane ion currents are characterized electrophysiologically in all filamentous fungi00:37
Our model system is membrane obtained from aerial hyphal cell – sporangiophore of Phycomyces blakeseeanus - 103:15
Our model system is membrane obtained from aerial hyphal cell – sporangiophore of Phycomyces blakeseeanus - 204:06
ORIC ≈ VRAC05:29
Without ATP ORIC rundown exponentially06:57
2mM intracellular ATP significantly slows ORIC rundown07:28
Mg2+ reduction further activates ORIC08:20
ATP modifies effects of ORIC inhibitors, A9C and NFA08:51
A9C and NFA are potent inhibitors of cell respiration and ATP synthesis10:40
Untitled11:24
Conclusions12:04