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Advanced STED microscopy of the membrane organisation in activating T-cells
Published on Jul 09, 2018511 Views
Nanoscale organization of the membranes of living cells plays crucial roles in numerous vital processes, including during the activation of T-cells and their formation of the immunological synapse. Ho
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Advanced STED microscopy of the membrane organisation in activated T-cells00:00
T-cells – key element of the immune system00:08
T-cell signalling00:41
T-cell triggering – Kinetic-segregation model - 101:16
T-cell triggering – Kinetic-segregation model - 203:01
Roles of the lipid environment in T-cell signaling?03:21
Membrane organisation in T-cells? - 104:44
Membrane organisation in T-cells? - 205:15
Lipid order? Polarity-sensitive probes05:58
Spectral sensitivity – pitfals of GP08:24
Maximising spectral sensitivity10:01
Lipid order in activated T-cells - 112:24
Lipid order in activated T-cells - 212:47
Lipid order in activated T-cells - 314:05
Does the membrane order correlate with the lipid diffusion rates?14:47
Fluorescence Correlation Spectroscopy (FCS)15:16
Scanning FCS16:34
Diffusion rates of PE-lipids in activated T-cells17:32
Do lipids diffuse freely at the sites of TCR / CD45?18:35
Lipid diffusion modes? Spot-variation STED-FCS - 119:10
Lipid diffusion modes? Spot-variation STED-FCS - 219:20
Diffusion modes of sphingomyelin (SM) in activated T-cells 20:28
Membrane properties of activated T-cells21:27
Triggering of T-cells on poly-L-lysine (PLL)22:20
Many thanks to …22:50