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Cutting edge biophysical tools to determine biomolecular interactions and characterize protein stability in solution
Published on Jul 09, 2018413 Views
Introduction to Micro Scale Thermophoresis Analysis of bio-molecular interactions, such as protein-protein, protein-nucleic acid or protein-small molecule, not only helps to develop therapeutics or di
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Chapter list
When binding affinity matters - 100:00
When binding affinity matters - 200:19
MicroScale Thermophoresis01:50
Theory of MST: Thermophoresis and TRIC02:25
Interactions accessible with MST03:17
Optical MicroScale Thermophoresis03:46
MST binding curve04:37
MicroScale Thermophoresis (MST) application slides05:38
Therapeutic IL1β antibodies05:39
Nitrate binding to a 12 transmembranehelices transporter07:00
GPCR-small molecule interactions08:15
The added value of MST 09:26
When protein stability matters.10:41
nanoDSF technology11:12
Aggregation detection with the backreflection technology12:27
How does the Prometheus NT.48 work?13:09
nanoDSF technology applications14:01
Comparison of technologies: nanoDSF - µDSC14:02
Detergent screens for integral membrane proteins15:29
PR.TimeControl - protein refolding kinetics and efficiency15:59
PR.ChemControl16:00
Benefits of nanoDSF16:01
Thank you for your attention17:16