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Mapping of hemoglobin residuals in erythrocyte ghosts using two photon excited fluorescence microscopy
Published on 2018-07-09362 Views
We have utilized the two photon excitation fluorescence (TPEF) microscopy to map the spatial distribution of residual hemoglobin. The home-made experimental set up for NLSM utilizes the train of the f
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Presentation
Hemoglobin imaging using two photon excitation fluorescence microscopy00:00
OUTLINE00:51
Why misrosocopy? - 102:05
Why misrosocopy? - 203:00
Why misrosocopy? - 303:28
Why misrosocopy? - 403:37
Why misrosocopy? - 503:43
Nonlinear effects - 104:07
Nonlinear effects - 204:09
Nonlinear effects - 305:31
Nonlinear effects - 405:47
Nonlinear effects - 507:03
Nonlinear effects - 607:22
Nonlinear effects - 707:28
Nonlinear effects - 807:32
Nonlinear effects - 907:53
Nonlinear effects - 1008:26
Nonlinear effects - 1108:36
Second and third harmonic generation - 109:04
Second and third harmonic generation - 210:20
Second and third harmonic generation - 311:10
Second and third harmonic generation - 411:30
Practical consequences of nonlinearity - 111:35
Practical consequences of nonlinearity - 213:02
Practical consequences of nonlinearity - 313:02
Recording process 13:16
From 2d to 3d image - 113:49
From 2d to 3d image - 214:16
From 2d to 3d image - 314:28
From 2d to 3d image - 414:41
Why nonlinear laser scanning microscopy (NLSM)? - 114:56
Why nonlinear laser scanning microscopy (NLSM)? - 215:04
Why nonlinear laser scanning microscopy (NLSM)? - 315:17
Why nonlinear laser scanning microscopy (NLSM)? - 415:19
Why nonlinear laser scanning microscopy (NLSM)? - 515:23
Why nonlinear laser scanning microscopy (NLSM)? - 615:34
Why nonlinear laser scanning microscopy (NLSM)? - 715:41
Why erythrocytes? - 115:53
Why erythrocytes? - 216:27
Why erythrocytes? - 317:13
Why erythrocytes? - 417:51
Motivation & state of the art - 118:32
Motivation & state of the art - 219:35
Motivation & state of the art - 319:48
Motivation & state of the art - 420:11
Motivation & state of the art - 520:31
Experimental setup - 120:36
Experimental setup - 220:39
Experimental setup - 321:01
Experimental setup - 421:08
Experimental setup - 521:21
Experimental setup - 621:25
Experimental setup - 721:27
Experimental setup - 821:48
Experimental setup - 921:50
Experimental setup - 1021:53
Experimental setup - 1122:00
Experimental setup - 1222:13
Human erythrocytes imaging - 122:15
Human erythrocytes imaging - 222:52
Porcine erythrocytes imaging - 123:01
Porcine erythrocytes imaging - 223:31
Imaging at single cell level23:35
After gradual hypotonic hemolysis - 123:59
After gradual hypotonic hemolysis - 224:25
After gradual hypotonic hemolysis - 325:03
Summary - 125:33
Summary - 225:42
Summary - 325:59
Summary - 426:06
Summary - 526:15
TPEF of chitinous structures - 126:17
TPEF of chitinous structures - 226:26
TPEF of chitinous structures - 326:28
Thank you for your attention!26:40