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Mapping of hemoglobin residuals in erythrocyte ghosts using two photon excited fluorescence microscopy
Published on Jul 09, 2018351 Views
We have utilized the two photon excitation fluorescence (TPEF) microscopy to map the spatial distribution of residual hemoglobin. The home-made experimental set up for NLSM utilizes the train of the f
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Hemoglobin imaging using two photon excitation fluorescence microscopy00:00
OUTLINE00:51
Why misrosocopy? - 102:05
Why misrosocopy? - 203:00
Why misrosocopy? - 303:28
Why misrosocopy? - 403:37
Why misrosocopy? - 503:43
Nonlinear effects - 104:07
Nonlinear effects - 204:09
Nonlinear effects - 305:31
Nonlinear effects - 405:47
Nonlinear effects - 507:03
Nonlinear effects - 607:22
Nonlinear effects - 707:28
Nonlinear effects - 807:32
Nonlinear effects - 907:53
Nonlinear effects - 1008:26
Nonlinear effects - 1108:36
Second and third harmonic generation - 109:04
Second and third harmonic generation - 210:20
Second and third harmonic generation - 311:10
Second and third harmonic generation - 411:30
Practical consequences of nonlinearity - 111:35
Practical consequences of nonlinearity - 213:02
Practical consequences of nonlinearity - 313:02
Recording process 13:16
From 2d to 3d image - 113:49
From 2d to 3d image - 214:16
From 2d to 3d image - 314:28
From 2d to 3d image - 414:41
Why nonlinear laser scanning microscopy (NLSM)? - 114:56
Why nonlinear laser scanning microscopy (NLSM)? - 215:04
Why nonlinear laser scanning microscopy (NLSM)? - 315:17
Why nonlinear laser scanning microscopy (NLSM)? - 415:19
Why nonlinear laser scanning microscopy (NLSM)? - 515:23
Why nonlinear laser scanning microscopy (NLSM)? - 615:34
Why nonlinear laser scanning microscopy (NLSM)? - 715:41
Why erythrocytes? - 115:53
Why erythrocytes? - 216:27
Why erythrocytes? - 317:13
Why erythrocytes? - 417:51
Motivation & state of the art - 118:32
Motivation & state of the art - 219:35
Motivation & state of the art - 319:48
Motivation & state of the art - 420:11
Motivation & state of the art - 520:31
Experimental setup - 120:36
Experimental setup - 220:39
Experimental setup - 321:01
Experimental setup - 421:08
Experimental setup - 521:21
Experimental setup - 621:25
Experimental setup - 721:27
Experimental setup - 821:48
Experimental setup - 921:50
Experimental setup - 1021:53
Experimental setup - 1122:00
Experimental setup - 1222:13
Human erythrocytes imaging - 122:15
Human erythrocytes imaging - 222:52
Porcine erythrocytes imaging - 123:01
Porcine erythrocytes imaging - 223:31
Imaging at single cell level23:35
After gradual hypotonic hemolysis - 123:59
After gradual hypotonic hemolysis - 224:25
After gradual hypotonic hemolysis - 325:03
Summary - 125:33
Summary - 225:42
Summary - 325:59
Summary - 426:06
Summary - 526:15
TPEF of chitinous structures - 126:17
TPEF of chitinous structures - 226:26
TPEF of chitinous structures - 326:28
Thank you for your attention!26:40