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Mechanically-driven ejection of viral DNA
Published on Jul 09, 2018399 Views
Viruses are parasitic infectious agents with a nanoscale shell, known as the capsid, that encapsulates the genomic material. Most bacteriophage viruses invade bacteria by transferring their genome ins
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Chapter list
Mechanically-driven ejection of viral DNA00:00
The T7 bacteriophage00:18
Life cycle of a dsDNA virus01:29
1. T7 nanomechanics05:58
Discrete steps occur in the 0-1.5 nN force range08:11
Similar discrete steps occur during mechanical relaxation10:22
Exploring the kinetics of the structural transition11:31
Mechanical buckling of the T7 capsid13:11
2. Temperature-dependent nanomechanics of T713:50
Temperature-dependent nanomechanics of T716:24
Temperature-dependent topography of T717:16
Temperature-dependent structural transitions of T720:40
3. Mechanically-driven ejection of T7 DNA21:14
Effect of force on the triggering of DNA ejection22:44
DNA ejection can also be triggered by glutaraldehyde (chemical fixation)24:15
DNA ejection triggered by a conformational switch which can be activated by fiber immobilization or mechanical force25:00
4. Perspectives25:40
Summary and acknowledgements27:49