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Biophysical modeling of bacterial immune system regulation
Published on Jul 09, 2018427 Views
Bacterial immune systems (CRISPR-Cas and restriction-modification systems) defend bacterial cells against invasion by viruses or plasmids. CRISPR-Cas has also found major biotechnology applications, b
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Chapter list
Biophysical modeling of bacterial immune system regulation 00:00
Bacterial immune systems00:33
Overview02:25
Modeling R-M system establishment03:35
R-M switch is a rudimental “immunological system”03:43
Bacterial Restriction-Modification (R-M) system05:08
Regulation by the control protein05:58
Quantitative model06:40
Comparison with the experiment07:24
Modeling restriction enzyme dynamics08:21
In-vivo R-M expression09:06
R and M expression dynamics09:46
Introducing population dynamics10:24
A very good agreement of the model with the data11:19
Identifying false regulatory mechanisms12:29
RM system design14:05
Dynamical property observables15:18
Perturbing any of the characteristic system features, diminishes some of the dynamical property observables. 16:02
Perturbing population dynamics16:40
R-M system summary17:32
Modeling CRISPR-Cas activation18:34
CRISPR/Cas system18:42
How is the system induced?20:08
CRISPR transcript processing20:31
Transcript processing model21:17
How to model transcription regulation?22:18
Transcripts concentrations at 20 min post-induction23:48
Perturbing pre-crRNA processing rate25:30
Conclusion 26:24
Acknowledgements28:19