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The multiple roles of Orai N-terminus in CRAC channel function
Published on Jul 09, 2018453 Views
Calcium (Ca 2+) is an essential second messenger required for diverse signaling processes in immune cells. Ca 2+ release-activated Ca 2+ (CRAC) channels represent one main Ca 2+ entry pathway into the
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Chapter list
The multiple roles of Orai N-terminus in CRAC channel function00:00
Introduction • Techniques • Results • Summary00:10
Ca2+ is a major factor regulating cellular processes00:25
Store-Operated-Channel (SOC)00:47
CRAC channel – STIM1 – Orai103:16
• Introduction • Techniques •Results • Summary • Patch Clamp Technique • Fluorescence Resonance Energy Transfer (FRET) Microscopy - 104:34
Activation of store-operated Orai1 channels04:48
STIM1 and Orai1 required for channel activity07:04
• Introduction • Techniques •Results • Summary • Patch Clamp Technique • Fluorescence Resonance Energy Transfer (FRET) Microscopy - 208:45
Förster Resonance Energy Transfer (FRET)08:47
• Introduction • Techniques • Results • Summary - Direct coupling of STIM1 to Orai1 results in Ca2+ channel activation10:24
STIM1 oligomerisation - 110:26
STIM1 oligomerisation - 210:58
STIM1 to Orai1 coupling - 111:51
STIM1 to Orai1 coupling - 212:09
STIM1 C-terminus is sufficient for ORAI1 activation13:00
STIM1-mediated ORAI1 conformational change - 114:04
STIM1-mediated ORAI1 conformational change - 214:30
STIM1-mediated ORAI1 conformational change - 315:56
STIM1 adjusts authentic CRAC channel hallmarks16:28
Orai1 C-terminus indispensable for binding to STIM118:37
Orai1 N-terminus essential for STIM1-mediated Orai1 gating19:37
Last 15aa of Orai1 N-terminus are indispensable for STIM1 mediated Orai1 gating - 120:46
Last 15aa of Orai1 N-terminus are indispensable for STIM1 mediated Orai1 gating - 221:00
Orai1 N-terminus mediates authentic CRAC channel characteristics22:06
Isoform specific Orai channel activation23:06
Orai1 N-terminus communicates with loop2 to mediate Orai channel gating - 123:39
Orai1 N-terminus communicates with loop2 to mediate Orai channel gating - 224:30
Orai1 N-terminus communicates with loop2 to mediate Orai channel gating - 325:03
Gating of Orai1 is further controlled via a communication of N-terminus with cholesterol - 125:59
Gating of Orai1 is further controlled via a communication of N-terminus with cholesterol - 226:29
Cholesterol binding is affected by a mutated Orai1 Nterminus26:37
Conclusion26:57
Thanks to:27:38