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Improving high-resolution optical imaging for (neuro-)biology
Published on Jul 09, 2018548 Views
Far-field optical super-resolution microscopy or nanoscopy techniques “super-resolve” features residing closer than the diffraction-limit by transiently preparing fluorophores in distinguishable (typi
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Chapter list
One example how to improve diffraction-unlimited imaging - 100:00
One example how to improve diffraction-unlimited imaging - 200:29
Protected STED: Improved long-term live-cell nanoscopy00:55
Coordinate-targeted nanoscopy with multiple off states - 101:09
Coordinate-targeted nanoscopy with multiple off states - 201:47
Reversible photoswitching02:00
Stimulated emission and reversible photoswitching02:27
Protection of fluorophores - 102:56
Protection of fluorophores - 203:17
Laser pulse sequence at each scan position -1 03:34
Laser pulse sequence at each scan position - 203:48
Vimentin cytoskeleton in living HeLa cell - 104:33
Vimentin cytoskeleton in living HeLa cell - 204:48
Vimentin cytoskeleton in living HeLa cell - 305:02
Synergistic action of two off-transitions05:09
Improved state contrast translates to improved resolving power - 105:31
Improved state contrast translates to improved resolving power - 206:17
Improved state contrast translates to improved resolving power - 306:22
Imaging with sub-diffraction resolution in all spatial directions - 106:55
Imaging with sub-diffraction resolution in all spatial directions - 207:22
Actin in living fibroblast07:34
Actin in living fibroblast: live labeling with nanobodies07:45
High-resolution 3D imaging in living brain tissue (slice culture)08:13
High-resolution 3D imaging in living brain tissue - 108:33
High-resolution 3D imaging in living brain tissue - 209:00
High-resolution 3D imaging in living brain tissue - 309:32
Home-built microscope09:46
Multiple off-state transitions10:04
Institute of Science and Technology Austria (Klosterneuburg) -1 10:28
Institute of Science and Technology Austria (Klosterneuburg) - 210:57
IST Austria: research across disciplines - 111:30
IST Austria: research across disciplines - 211:43
IST Austria12:01
Molecular architecture of biological systems - 112:11
Molecular architecture of biological systems - 212:43
Molecular architecture of biological systems - 313:03
Neuroscience questions - 113:19
Neuroscience questions - 2 13:35
Neuroscience questions - 313:37
Neuroscience questions - 413:43
First STED data from lab at IST Austria - 114:12
First STED data from lab at IST Austria - 214:29
Nanopatterns resolved via STED microscopy - 114:37
Nanopatterns resolved via STED microscopy - 214:46
First data from IST: living brain slice15:04
First data from the lab at IST Austria - 115:25
First data from the lab at IST Austria - 215:55
First data from the lab at IST Austria - 315:59
X10 Expansion Microscopy, trans-synaptic organization - 116:08
X10 Expansion Microscopy, trans-synaptic organization - 216:40
Deep tissue STED microscopy - 116:57
Deep tissue STED microscopy - 217:09
Deep tissue STED microscopy - 317:29
Institute of Science and Technology Austria (Klosterneuburg) - 117:43
Institute of Science and Technology Austria (Klosterneuburg) - 217:51
Institute of Science and Technology Austria (Klosterneuburg) - 318:08