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Identification of microorganisms by mass spectrometry
Published on Jul 09, 2018407 Views
Currently used techniques for species identification are mostly relied on protein database matching or alternatively on immunological procedures. Although widely used, such an approach does not always
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Chapter list
Identification of microorganisms by mass spectrometry - 100:00
Identification of microorganisms by mass spectrometry - 200:42
Biotypization means species identification01:01
e.g. pathogenic Escherichia coli03:11
The first complete DNA sequence of an E. coli genome(laboratory strain K12 derivative MG1655) was published in 1997 - 104:48
The first complete DNA sequence of an E. coli genome(laboratory strain K12 derivative MG1655) was published in 1997 - 207:00
POLIMERASE CHAIN REACTION (PCR) species determination 08:20
How to determine the difference?10:30
Clinical practice- One day12:27
MALDI MASS SPECTROMETRY species determination14:38
Bruker MALDI BioTyper Workflow16:18
LOW RESOLUTION MALDI MASS SPECTROMETRY18:01
The reference library now includes more than 2,700 species of 458 microorganism genera.18:56
Is there any other concept?19:19
PROTEIN READING - 120:19
PROTEIN READING - 221:16
Sample preparation workflow22:16
PROTEIN READING - 323:03
PROTEIN READING - 423:58
PROTEIN READING - 524:34
HIGH RESOLUTION MALDI MASS SPECTROMETRY species determination25:11
PROTEIN READING - 625:38
1-D chromatography on bacterial peptides from fish (45 min separation)26:58
Common and rare clinical strains28:08
Untitled28:21
2-D chromatography on bacterial peptides up to the subspecies level28:36
COMPARISON WITH OTHER MS BIOTYPIZATION APPROACHES29:20
Conclusions29:52
Welcome! 14th International School of Biophyscis31:57