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The study of molecular disease profiles with mass spectrometry based molecular imaging approaches
Published on Mar 10, 20142465 Views
The study of molecular signaling processes related to disease requires not only the detection and analysis of the molecules involved but also the evaluation of their spatial organization. Many molecul
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The study of molecular disease profiles with mass spectrometry based molecular imaging approaches00:00
Rembrandt´s painting02:23
The study of disease…03:13
From van Leeuwenhoek…03:59
To molecular pathology with MS04:33
Molecular basis for histology06:11
At the interface of disciplines06:50
Molecular Man09:20
MS based disease profiling10:25
Macromolecular desorption and ionization - 110:29
Macromolecular desorption and ionization - 211:24
Microprobe imaging MS12:29
Microscope mode imaging MS12:48
Microprobe vs Microscope14:32
Molecular fingerprints15:04
A multimodal approach15:56
Tumor metabolism elucidated17:19
Three areas of innovation - 118:15
Three areas of innovation - 219:04
Confused? That’s a good thing!19:35
Based on Peter Higgs´s work20:22
Timepix: A pixelated protein camera - 120:56
Timepix: A pixelated protein camera - 221:31
Seeing intact antibodies!21:56
Mutli modal X-ray CT23:51
Arthtritis24:14
Arthritis schematics24:57
Proteome imaging protocol25:58
MALDI‐MSI experiments28:27
PCA‐DA targeting practice29:05
Classification of OA29:25
OA markers localized and identified29:40
Fibronectin tryptic peptides30:05
Complementary ImmunoHistoChemistry30:40
Quantitative evaluation31:06
OA marker protein inventory31:20
Potentially new targets31:33
Small molecules in superficial layer32:25
Mineral deposits in OA cartilage33:30
Brainpath34:25
Brainpath workflow34:33
Primary metabolites in MFP2 KO model36:06
MALDI images MFP2 model37:05
Selected area PCA. Target = cerebellum37:34
Distinctive peaks: PCA loadings37:50
Corresponding SIMS FA images38:06
PCA Images39:07
HCA reveals FA accumulation in Int.Nucl.39:20
MSI co registration with Allan Brain Atlas39:29
Complexity…40:53
Heterogeneity - 141:16
Nothing is the same…41:18
Dealing with heterogeneity…41:56
Heterogeneity - 243:05
Protein differentiation43:29
Pathway analysis: from cells to tissue44:58
Increasing throughput46:21
Tissue differentiation in TMA’s…47:51
ToF‐SIMS PCA‐CorClus of TMA48:20
Histology vs. Imaging MS48:40
What do we learn?49:30
Summary50:41
Acknowledgements51:16
Positive ion TOF-SIMS spectrum of an embedded paint cross section57:43